2 edition of Microscopial methods for determining the organisation of the pollen tube cytoskeleton found in the catalog.
Microscopial methods for determining the organisation of the pollen tube cytoskeleton
Fiona P. Doris
|Statement||by Fiona P. Doris.|
|Contributions||University College Dublin. Department of Botany.|
|The Physical Object|
|Number of Pages||186|
to reach the egg. The detailed organization of the pollen tube cytoskeleton in vivo is not known. In vitro, the vegetative nucleus migrates within the elongating tube in a network of microfilaments and microtubules (Lazzaro , ). The spermatogenous cell re-mains within the grain in vitro, enmeshed in a network. The opening lecture by Professor Jack Heslop-Harrison (–) was focused on organelle transport in pollen tubes (‘The pollen tube: motility and the cytoskeleton’) and on the use of video-enhanced microscopy to correlate the movement of pollen tube organelles with the organization of actin filaments and microtubules (Heslop-Harrison Cited by:
Journal of Cell Science publishes cutting-edge science, e ncompassing all aspects of cell biology.. The journal is led by Editor-in-Chief Michael Way and a prestigious team of Editors who are research-active academics and leaders in their respective fields; they are supported by an outstanding Editorial Advisory Board that reflects all relevant areas in cell biology, including recently. Using this method with pollen from other species, Butler published a list of the best flowers and the best concentrations to use throughout the spring and summer. You will find this in Butler K.G. "Pollen germination across the seasons" School Science Review 82 () p93f.
competitive pollen tube growth takes place in the pistil. In higher plants, pollen tube growth is highly polarized and extremely fast. Indeed, the pollen tube is the fastest growing plant cell known. Rates of pollen tube growth can reach mms¡1 in various plant species (e.g. mms¡1 in maize and mms¡1 in lily, cal-. Apple pollen tube growth model • Predicts how long it takes for flowers of different cultivars to be fertilized after they has been pollinated based on ambient temperature • This information can help inform crop load management decisions, especially timing of chemical bloom thinners (no .
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The cytoskeleton in pollen tubes has been intensively studied, because of its abundance and prominent roles and because the pollen tube is an excellent experimental system for cell biological studies. Pollen actin microfilaments (MFs) exist as multiple distinct populations, each participating in a specific cellular trafficking or organization by: Here we focus on the organization of microtubules and on their putative role in the transport of pollen tube organelles.
We will discuss the model of functional cooperation between microtubules and actin filaments and adapt it to the pollen tube system. Results from pollen tube studies provide an excellent example of efforts to more accurately depict actin organization and from which lessons can be learned for studies on actin in root cells.
Pollen tubes are characterized by growth that is focused toward the extreme apex of the cell and actin polymerization has been shown to play an integral Cited by: The Microtubule Cytoskeleton and Pollen Tube Golgi Vesicle System are Required for in Vitro S-RNase Internalization and Gametic Self-Incompatibility in Apple The methods used were according to.
Organelle movement and fibrillar elements of the cytoskeleton in the angiosperm pollen by: The method relies on Congo Red binding to pollen tubes and correlates dye fluorescence to tube length. This method can be used in any laboratory without specialized equipment, and has the potential to enable larger screens as chemical libraries grow and to make chemical screening accessible to researchers building specialized libraries designed to probe pathways in plant by: 2.
Pollen tubes grow rapidly in a strictly polarized manner as they transport male reproductive cells through female flower tissues to bring about fertilization. Vegetative pollen tube cells are an excellent model system to investigate processes underlying directional cell by: 7. INTRODUCTION. Pollen tube growth delivers the sperm to the ovule in higher plants and is thus essential for sexual reproduction.
The process is highly polarized, fast, and dependent on the actin cytoskeleton (Frankeet al., ; Mascarenhas and Lafountain, ; Steer and Steer, ; Derksen et al., ; Taylor and Hepler, ), although the mechanism is still controversial (Vidali and.
Pollen tube growth is an essential step for successful plant reproduction. Vacuolar trafficking and dynamic organization are important for pollen tube growth; however, the key proteins involved in these processes are not well understood. Here, we report that the ADAPTOR PROTEIN-3 (AP-3) complex and its tonoplast cargo PROTEIN S-ACYL TRANSFERASE10 (PAT10) are critical for pollen tube Cited by: 5.
Pollen Methods Course Brown University June 7 – 12, MEASURING POLLEN TUBE LENGTH WITH IMAGEJ process it is best to determine this volume empirically. Once the PGM is at pH it is ready for us in liquid culture. When making solid media add % to 2% low melt agarose to pollen File Size: 6MB.
To determine if the F-actin-severing activity of MDP25 plays a role in the organization and dynamics of actin filaments in pollen tubes, we observed the actin cytoskeleton. To visualize actin organization and dynamic features in pollen tubes, we used an actin filament marker lifeact-mEGFP, which is driven by the pollen-specific promoter LatCited by: Pollen germinated (%) and grew pollen tubes satisfactorily ( μm) within the range of % sucrose, whereas with the addition of % PEG, pollen germinated, but tube.
Pollen morphology, pollen germination percentage, pistil receptivity and embryo development were investigated using the in vitro culture method, the paraffin section technique, scanning electron microscopy and transmission electron microscopy. An important component of the signal transduction mechanism are ion flux, intracellular ion gradients and dynamics, which are critical for the polarization of pollen tubes and for maintaining the growth site at the tube tip.
The actin-binding protein-mediated organization of the actin cytoskeleton within the pollen tube plays a crucial role in this polarized process. However, the mechanism underlying the polarity of the actin filament (F-actin) array and behaviors in pollen tube growth remain largely by: 7.
Chemical signaling for pollen tube guidance at a glance Yoko Mizuta1,2,* and Tetsuya Higashiyama1,3 the tip in a manner that is controlled by cytoskeleton dynamics (Cheung and Wu, ). The pollen tube grows through the pistil method to co-cultivate and analyze pollen tubes and ovules on solid medium.
Pollinated stigma is cut and Cited by: In pollen microtubule-associated proteins (MAPs) in the tubes, four different MT-associated motor proteins Pollen Tube Cytoskeleton have been identified by biochemical and cell biologi- prising because there are at least seven kinesin- cal tools: two kinesins, a pollen tube kinesin homo- related genes in A.
thaliana. logue (PKH) (Cai and. Molecular Plant • Pages 1–17, The Dynamic Pollen Tube Cytoskeleton: Live Cell Studies Using Actin-Binding and Microtubule-Binding Reporter Proteins Alice Y. Cheunga,b,c,1, Qiao-hong Duana, Silvia Santos Costad,e, Barend H.J. de Graafa,f, Veronica S. Di Stilioa,g, Jose Feijod,e and Hen-Ming Wua,b a Department of Biochemistry and Molecular Biology.
Biography. Rui Malho is an associate professor in the Department of Plant Biology, Faculty of Sciences of Lisbon. He holds a Ph.D.
in cell biology, with a major interest in signal transduction mechanisms and imaging of cell dynamics--in particular, the study of pollen tube growth and its signalling pathways.
S-RNase is the female determinant of self-incompatibility (SI) in pear (Pyrus bretschneideri). After translocation to the pollen tube, S-RNase degrades rRNA and induces pollen tube death in an S -haplotype-specific manner. In this study, we found that the actin cytoskeleton is a target of P.
bretschneideri S-RNase (PbrS-RNase) and uncovered a mechanism that involves Cited by:. Pollen tubes extend rapidly when pollen grains are incubated in defined media. Tube extension requires many critical functions of plant cells including molecular signaling, cytoskeleton remodeling, secretion, and cell wall synthesis.
Consequently, pollen Cited by: 2.The pollen tube of most seed plants acts as a conduit to transport sperm cells from the pollen grain, either from the stigma (in flowering plants or angiosperms) to the ovules at the base of the.Pollen germination and pollen-tube growth.
International Review of Cytology Heslop-Harrison J, Heslop-Harrison Y, Cresti M, Tiezzi A, Moscatelli A. Cytoskeletal elements, cell shaping and movement in the angiosperm pollen tube. Sci. Heslop-Harrison J. The pollen tube: Motility and the cytoskeleton.